LTMT JRS fellow, Awardee of DST INSPIRE fellowship, Doctoral student, Budding_Virologist, Gamer😁
Shimoga, India
Joined June 2023
- Tweets917
- Following161
- Followers49
- Likes4.8K
Amod Raghupathi Bhat retweeted
Kindly amplify:
The application for SIB-SHInE Program 2026 is now open - If you are passionate about engineering for healthcare this maybe your big opportunity.
Please apply - docs.google.com/forms/d/e/1F…
Feel free to ask me any question you may have regarding this program.
@ATinyGreenCell Fairly large plasmid (lane 2 and 3) about 15.5kb lane 4 9kb plasmid. Classical alkaline lysis and I loaded bit more in lane 3. I am not able to understand what that band is which is marked in RED. Is it ssDNA ? What are those faint bands above the red one?
@HemanthGovinde and me, We both extracted this from a 50ml culture and the elute was 1 ml out of which I loaded 5 ul. Help us out.
Amod Raghupathi Bhat retweeted
I got hit by some rather sudden and extreme financial hardship so if anyone is in need of remote wetlab contract research, strictly BSL1, do let me know. Currently scrambling for gigs.
Plant, Bacterial, Archaeal Bioeng
Custom Lab Hardware
Turn Key Genetic Design
Please RT 💚
I desperately needed help to find a plasmid that is low copy number and should support mammalian expression. I have been struggling for one year to clone a flavivirus replicon and I m out of luck. Please help. Tags for reach and support @abhijit_MLab @abandopa @ATinyGreenCell
@patankar_lab @RajeshPandeyLab I follow very few labs in Twitter. Forgive for being naive and tagging you all. Any help will be really appreciated.
Thank you in advance #AcademicTwitter #PhDlife
Amod Raghupathi Bhat retweeted
There is a calculated and thorough attack on the federal structure and languages of india by @RajbhashaVibhag . It wants India to be #Hindia since independence.
We Kannadigas will fight back hindi imperialism.
No,
HINDI IS NOT OUR NATIONAL LANGUAGE
WE WILL NOT ACCEPT HINDI BECAUSE OF SANSKRIT SEEPAGE
SANSKRIT IS NOT THE MOTHER OF KANNADA
HINDI/URDU ARE ONE AND THE SAME.
KANNADA IS NATIONAL LANGUAGE IN KARNATAKA.
All 22 languages under schedule 8 are national languages.
#StopHindiImposition
#StopHindiImperialism
#StopHindiDiwas
#KannadaLanguagePlanning
Amod Raghupathi Bhat retweeted
ಹಿಂದಿ ಹೇರಿಕೆ ನಿಲ್ಲಿಸಿ
#StopHindiImposition
#StopHindiDiwas
@ATinyGreenCell Hey do u think a secondary structure of free energy -2kcal/ mole will inhibit hifi builder assembly?
I tried to assemble two fragments of size 3.2kb each in 10.6kb vector. I scaled down the reaction to 10 ul and I ran for 60min to check if assembly occurred and I ran 5ul on gel. Could not see the assembled product.
Homology is 20bp.
Tm of homology is 58-60degree.
@ATinyGreenCell Update! Performed alkaline lysis with your protocol. I could not eliminate the gdna, but it was way lesser than wat I usually get!
I also performed the digest to the same and purified the fragment with PEG! Worked once again beautifully. Can't thank you enough😁
Alkaline lysis. This was scaled up to 25ml culture.
Elite was 60ul and loaded 2 and 4ul in lane 1 and 3 respectively.
Lane 1 and 3: 10.6kb vector.
Lane 2 1kb ladder
