Jinyan retweeted
More on copper catalysis in nonheme enzymes: we have engineered hydroxymandelate synthase to catalyze alkene oxytrifluoromethylation, generating a broad range of chiral trifluoromethyl-containing β-, γ-, and δ-lactones with high enantioselectivity (pubs.acs.org/doi/full/10.102…). This work is led by graduate student James Zhang (@zhangg_james) A key highlight of this work is that the reaction proceeds exclusively with a copper center, whereas the same enzyme scaffold with an iron center catalyzes alkene trifluoromethyl azidation. These features underscore how the identity of the metal center can unlock distinct and diverse catalytic functions in nonheme enzymes for non-natural tansformations.
Jinyan retweeted
Another exciting milestone in biocatalysis with nonheme enzymes! We have developed the first enzymatic system for copper-catalyzed radical C(sp³)–N coupling: science.org/doi/full/10.1126…, which also represents the first demonstration of employing pterin-dependent nonheme enzymes in non-natural reactions. This work was led by postdoctoral fellow Dr. Xuzhong Shen (@lanser1208 ), in collaboration with Prof. Yi Rao’s group at Utah State University, Prof. Yunfang Yang at Zhejiang University of Technology, and Dr. Marc Garcia-Borràs @MarcGBQ from the University of Girona. Once again, this study highlights the power of combining metal substitution with nonheme enzyme biocatalysis to push the boundaries of biocatalytic transition metal catalysis.
Amazing work! Congratulations!🥳
After a long search, we have finally achieved cross-coupling chemistry with nonheme enzymes (doi.org/10.26434/chemrxiv-20…)! In this preprint, led by graduate student Xiuze Wang (@XiuzeWang) with important contributions from postdoctoral fellow Dr. Xianhai Tian, we describe the integration of ligand-to-metal charge transfer (LMCT) photocatalysis, metal substitution, and nonheme enzyme biocatalysis to reengineer ethylene-forming enzyme (EFE) for nickel-catalyzed C(sp²)–S cross-coupling. The evolved enzyme catalyst accepts a broad range of thiols and aryl halides, enabling the synthesis of more than 50 diverse thioether products. Collaborations with my colleague Art Bragg, Prof. Yunfang Yang at Zhejiang University of Technology, and Dr. Marc Garcia-Borràs @MarcGBQ from the University of Girona, provided critical mechanistic insights into this process. This study marks our first step into enzymatic cross-coupling chemistry. Stay tuned for more exciting advances from our lab in nickel catalysis!
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Jinyan retweeted
After a long search, we have finally achieved cross-coupling chemistry with nonheme enzymes (doi.org/10.26434/chemrxiv-20…)! In this preprint, led by graduate student Xiuze Wang (@XiuzeWang) with important contributions from postdoctoral fellow Dr. Xianhai Tian, we describe the integration of ligand-to-metal charge transfer (LMCT) photocatalysis, metal substitution, and nonheme enzyme biocatalysis to reengineer ethylene-forming enzyme (EFE) for nickel-catalyzed C(sp²)–S cross-coupling. The evolved enzyme catalyst accepts a broad range of thiols and aryl halides, enabling the synthesis of more than 50 diverse thioether products. Collaborations with my colleague Art Bragg, Prof. Yunfang Yang at Zhejiang University of Technology, and Dr. Marc Garcia-Borràs @MarcGBQ from the University of Girona, provided critical mechanistic insights into this process. This study marks our first step into enzymatic cross-coupling chemistry. Stay tuned for more exciting advances from our lab in nickel catalysis!
Jinyan retweeted
I am excited to share our recent paper on engineering nonheme enzymes for copper-catalyzed Conia-ene reactions: nature.com/articles/s41929-0…. This work was pioneered by postdoc Xinpeng Mu and newly graduated Dr. Xinyuan Ji @xyji15. This milestone highlights the power of applying metal-substitution strategy in nonheme enzyme scaffold to create new biocatalytic reactions. Stay tuned for more exciting copper biocatalysis from our laboratory!
Access the free text here: rdcu.be/euNsb
Happy to see my second paper published🥳We’ve taken an important step forward in metalloenzymatic catalysis by integrating synthetic photocatalysts with non-heme iron enzymes to unveil a new mechanism for radical azidation and thiocyanation. Huge thanks to all our collaborators☺️
I am thrilled to share our recent Nature Catalysis cover-featured paper on engineering nonheme iron enzymes for metallophotoredox catalysis, enabling decarboxylative C-N₃ and C-SCN bond formation nature.com/articles/s41929-0…. This work was pioneered and led by graduate student Jinyan Rui @JRui7001, with important contributions from postdoc Dr. Xinpeng Mu. It has been a pleasure collaborating with the Marc Garcia-Borràs group @MarcGBQ and Yisong Guo's group @MossbauerLab, who provided valuable insights into the mechanistic aspects of this system using computational and spectroscopic tools. This milestone marks our first venture into photoredox catalysis with metalloenzymes and highlights the remarkable versatility of nonheme enzymes in driving reactions previously unknown in enzymatic catalysis.
Access the free text here: rdcu.be/d4kvT
Check out this research briefing from our group for further insights into this work: nature.com/articles/s41929-0…
Wishing you all a joyful holiday season! 🎄✨
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Jinyan retweeted
Photobiocatalysis with non-haem iron enzymes for enantioselective radical transformations nature.com/articles/s41929-0…
Jinyan retweeted
Biocatalytic Generation of CF3 Radicals by Nonheme Fe Enzymes for Enantioselective Alkene Difunctionalization | JACS @CmuScience @CarnegieMellon #Biocatalysis #Trifluoromethyl #Radical #Nonheme #Fe #Enzyme #Alkene #Difunctionalization @TotalSyntheses pubs.acs.org/doi/10.1021/jac…
Jinyan retweeted
Both my group and Yang's group @yang2biocat have demonstrated that nonheme iron enzymes can be engineered for enantioselective C(sp3)-F bond formation via iron-mediated radical fluorine transfer. Huge congratulations to the team led by postdoc Qun Zhao @qun_zhao , now a professor at Jiangnan University, and Dr. Zhenhong Chen @noelnehc, who just completed his thesis defense (the first in my lab) and has commenced his journey at Merck.
Our paper can be accessed through this link for free on Nature Synthesis rdcu.be/dCLc5, and you can find Yang's work on ChemRxiv chemrxiv.org/engage/chemrxiv…
Our lab has successfully engineered a non-heme iron enzyme for C-F bond formation via a radical pathway. 🎉🥳Check this paper out here for a free view-only version: rdcu.be/dCLc5
Now online:
Article by Yunfang Yang, @MarcGBQ, @xiongyih & co-authors
Engineering non-haem iron enzymes for enantioselective C(sp3)–F bond formation via radical fluorine transfer
nature.com/articles/s44160-0… ($)
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Jinyan retweeted
Now online:
Article by Yunfang Yang, @MarcGBQ, @xiongyih & co-authors
Engineering non-haem iron enzymes for enantioselective C(sp3)–F bond formation via radical fluorine transfer
nature.com/articles/s44160-0… ($)
2022 is a special year to experience lots of things. I learned to appreciate the beauty of life in its different seasons and colors. So I saw amazing views that I never had before. These memories will continue to give me courage for 2023 ✨ Happy New Year! 🎆
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Very exciting to see our paper getting highlighted on this special issue!!
Our October issue marks the start of a special virtual initiative focusing on #biocatalysis research! Cover by @Rosie_MC1R with contributions also by @AChemSynBio @PabloINik @JCLewisLab @deMelloGroup...Read it here cell.com/chem-catalysis/curr… and submit your #biocat paper!
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Jinyan retweeted
A radical-relay approach to biocatalytic C–N bond formation by @jacktennantchem and @KFBChem at @ASU_SMS and @ASUBiodesign in @ChemCatalysis cell.com/chem-catalysis/full…
Jinyan retweeted
It was a pleasure to contribute a preview article as part of a special issue on biocatalysis in @ChemCatalysis @CellCellPress. In this article, we highlight the incredible work by @xiongyih and team on a radical-relay approach to C-N bond formation! cell.com/chem-catalysis/full…
Our October issue marks the start of a special virtual initiative focusing on #biocatalysis research! Cover by @Rosie_MC1R with contributions also by @AChemSynBio @PabloINik @JCLewisLab @deMelloGroup...Read it here cell.com/chem-catalysis/curr… and submit your #biocat paper!
Jinyan retweeted
Very pleased to announce my first publication! Many thanks to the one and only @KFBChem for the guidance in writing this as well as to the group of @xiongyih for developing such a remarkable approach to biocatalytic C-N bond formation!
Check it out here:
cell.com/chem-catalysis/full…
This is my first paper and our lab’s first one! For me, it’s a great honor to accomplish this work and be a part of this amazing team. Tremendous thanks to Xiongyi for his encouraging and insightful guidance! I also appreciate all the help and support from my collaborators! :)
Excited to share my lab’s first paper online today at Science. So proud of my team @JRui7001 @qun_zhao @ajhuls1 @noelnehc It was great fun to collaborate with Guo Group @MossbauerLab at CMU and Garcia-Borràs Group @MarcGBQ at IQCC-UdG on this project! science.org/doi/10.1126/scie…
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