The facility focuses on stem cell engineering and cell fate determination, supporting collaborative projects using human pluripotent stem cell systems.
New York, NY
Joined October 2024
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SKI Stem Cell Research Facility retweeted
Online Now: Applying #CRISPR activation to verify single-/dual-reporter hPSC lines, dTAG #knockins & silent gene #knockouts. A rapid, effective assay without the need for differentiation to induce target gene expression.
ow.ly/pVtA50VPtow
@MSKCancerCenter @ISSCR
SKI Stem Cell Research Facility retweeted
Beautiful work by the Stadtfeld and @TommyVierbuchen labs on the role of genetic variation on imprinting and dna methylation stability in mouse pluripotent stem cells cell.com/stem-cell-reports/f…
SKI Stem Cell Research Facility retweeted
We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their DNA targets. science.org/doi/10.1126/scie…
Happy long weekend！
This is a quick message:
PE-Plus integrates MLH1dn and p53DD, which together inhibit the mismatch repair and p53 pathways; while PE7 incorporates La, a factor involved in various aspects of RNA metabolism. Both PE-Plus and PE7 are built on PEmax.
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For electroporation experiments, we recommend using PE7 directly, as it is currently the most efficient prime editing version. By the way, co-delivery of p53DD (Addgene Plasmid #214080) can further enhance prime editing efficiency when using PE7!
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We now offer various inducible PE hPSC lines, including iPE2, iPE4, iPEmax, iPE-plus, and iPE7 (we recently generated and tested it!) for both internal and external researchers, and welcome to collaborate!
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Excited to share our “Robust and inducible genome editing via an all-in-one prime editor in human pluripotent stem cells," is now published @NatureComms
nature.com/articles/s41467-0…
Potential applications:
1.Highly efficient precise mutation induction/correction in hPSCs without the DSB and donor template.
2.Convenient to generate multiple mutations in a single hPSC line in ONE step.
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The plasmids used for the iPE line generation, lenti-ePegRNAs and the backbone have been deposited at Addgene:
addgene.org/browse/article/2…
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The study was led by our Senior scientist Dr. Youjun Wu and collaborated with Studer lab @TaeWanKim @studerl. We introduced the iPE-Plus platform, enabling efficient and controllable multiplex editing in hPSCs.
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SKI Stem Cell Research Facility retweeted
Pleased to share our story @NatureComms on the discovery of genes regulating #hPSC pluripotent identity versus cell fitness through genome-scale CRISPR-Cas9 KO screens, as part of the #MorPhiC initiative aiming at establishing the function of human genes. rdcu.be/dXi72
ALT Parallel genome-scale CRISPR-Cas9 screens uncouple human pluripotent stem cell identity versus fitness by Bess Rosen and Danwei Huangfu
SKI Stem Cell Research Facility retweeted
👏 Congratulations to Dr. Deb Schrag, Chair of the @MSK_DeptOfMed, and Dr. @studerl, Director of the Center for Stem Cell Biology at MSK, who were elected to the @theNAMedicine, one of the highest honors bestowed upon scientists worldwide.
Learn more: bit.ly/4fvwkoP
On Oct 7- 11, we will conduct the hands-on training that teaches basic hPSC culture techniques. Topics covered include: hPSC thawing, expansion, passaging, freezing, and solving some technic questions/issues you may have. Reach out [email protected] for more info. Welcome to join!
SKI Stem Cell Research Facility retweeted
Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells biorxiv.org/cgi/content/shor… #biorxiv_cellbio