Philipp Steen (@PhilippSteen) header
Philipp Steen (@PhilippSteen) profile picture

Philipp Steen

@PhilippSteen

Physics PhD student in the @JungmannLab @MPI_Biochem @LMU_Muenchen @IMPRS_ML. Pushing the limits of microscopy!

Joined July 2020
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Photo by Philipp Steen (@PhilippSteen)
How do you scale super-resolution microscopy to dozens of proteins without linearly scaling imaging time? We introduce Combi-PAINT: a combinatorial DNA-PAINT strategy that breaks the 1-target-per-round bottleneck of Exchange-PAINT: biorxiv.org/content/10.64898… 1/5
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Bonus: We’re not the only ones to come up with an idea like this – @BalzarottiFran has developed a closely related technique, so we’re extra confident about applicability and reproducibility! 4/5
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Here’s their paper: biorxiv.org/content/10.64898… And our work: biorxiv.org/content/10.64898… Many thanks to @JungmannLab, all the co-authors and everyone who partook in this project! 5/5
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Out today from the @JungmannLab! A comprehensive comparison of how 18 different dyes perform in DNA-PAINT along with optimized guidance for dye selection and multiplexed imaging. nature.com/articles/s41592-0…
Image from Nature Methods (@naturemethods)
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Check out the Tweetorial here to get an overview!
Jungmann Lab (@JungmannLab) profile pictureJungmann Lab@JungmannLab7 Aug 2024
What makes for a great DNA-PAINT experiment? How does the fluorescent dye affect the image quality? And how can we use this to extract the most out of our samples? Check out our paper in @NatureMethods to see what state-of-the-art DNA-PAINT can do for you! doi.org/10.1038/s41592-024-0…
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What is a great DNA-PAINT experiment? How does the fluorescent dye affect the image quality? And how can we extract the most information from our samples? Check out our paper in @naturemethods to see what state-of-the-art DNA-PAINT can do for you! nature.com/articles/s41592-0…
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Philipp Steen retweeted
Jungmann Lab (@JungmannLab) profile picture
Jungmann Lab@JungmannLab28 Sep 2023
Big congrats to @SusanneReinhar3 and @PhilippSteen for winning the Student Award at this year’s PicoQuant single molecule workshop #WS28. Terrific job, very proud of both of you!!!
Image from Jungmann Lab (@JungmannLab)
Image from Jungmann Lab (@JungmannLab)
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Congratulations to all of our 2023 MPIB Junior Scientists' Publication Award winners. You have done outstanding work!
Image from Max Planck Institute of Biochemistry (MPIB) (@MPI_Biochem)
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Philipp Steen retweeted
Luciano A. Masullo (@l_masu) profile picture
Luciano A. Masullo@l_masu24 May 2023
ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY. Can we do structural biology with an optical microscope? Yes! Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out today in @Nature! nature.com/articles/s41586-0… @JungmannLab @MPI_Biochem
Ångström-resolution fluorescence microscopy

Ångström-resolution fluorescence microscopy

Nature - The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the...

nature.com
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Ångström-resolution fluorescence microscopy! We’re extremely happy to introduce resolution enhancement by sequential imaging (RESI). Have a look! @SusanneReinhar3 @l_masu @IBaudrexel @JungmannLab nature.com/articles/s41586-0…
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Philipp Steen retweeted
Luciano A. Masullo (@l_masu) profile picture
Luciano A. Masullo@l_masu5 Apr 2023
#FOM2023 coming to an end, excellent and inspiring talks and posters, amazing methods and applications! Hope you liked our contributions on #RESI and #DNAPAINT from the Jungmann Lab! @SusanneReinhar3 @PhilippSteen – at Oporto, Portugal
Image from Luciano A. Masullo (@l_masu)
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Big fields of view (FOV) for #DNAPAINT! In single-molecule localization microscopy, Gaussian illumination limits the usable FOV. By adding a piShaper to the excitation path, @l_masu and I create a beam with a flat-top profile, achieving even illumination across the camera 🧬🔬
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This corresponds to ~150 μm x 150 μm in the sample. A resolution of ~5 nm is achieved throughout the FOV. The design is adapted from Stehr & Stein et al. (nature.com/articles/s41467-0…).
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