Sebastian Barthel (@SebBarthel) profile picture

Sebastian Barthel

@SebBarthel

Postdoctoral researcher @erblabs (MPI Marburg)

Joined January 2018
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  • Followers79
I am happy to share our pre-print on the genetically encoded control of in vitro DNA replication in the PURE system, including data comparing in vitro system setups and highly active phi29 DNAP mutants. Check it out! A big thank you to everybody @erblabs! t1p.de/e4yhl
Genetically encoded control of in vitro transcription-translation coupled DNA replication

Genetically encoded control of in vitro transcription-translation coupled DNA replication

The bottom-up reconstruction of cellular functions has gained increasing attention for studying biological complexity, and for developing advanced biotechnological tools, including synthetic cells. A...

biorxiv.org
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🚨 Paper Alert 🚨 We are excited that our work on controlling in vitro DNA replication in a reconstituted transcription-translation system (TTcDR) by using allosteric transcription factors has now been published in ACS Synthetic Biology! tinyurl.com/cvrtkkrn Check it out! :)
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Sebastian Barthel retweeted
Luca Schulz (@schulluc) profile picture
Luca Schulz@schulluc21 Nov 2024
New in @EMBOjournal, we studied the evolution of essentiality in Form I Rubisco. While @KaHochberg nicely summarizes the evolutionary implications of our work, let me summarize some of the cool biochemistry surrounding it. doi.org/10.1038/s44318-024-0… 🧵
Layered entrenchment maintains essentiality in the evolution of Form I Rubisco complexes

Layered entrenchment maintains essentiality in the evolution of Form I Rubisco complexes

The EMBO Journal - Protein complexes composed of strictly essential subunits are abundant in nature and often arise through the gradual complexification of ancestral precursor proteins....

link.springer.com
Georg Hochberg (@KaHochberg) profile pictureGeorg Hochberg@KaHochberg21 Nov 2024
New paper from us and @erblabs by @schulluc : embopress.org/doi/full/10.10…. It's about the evolution of essentiality. This question has fascinated me for a long time: How does a component that was previously unnecessary for some function become essential for it?
Layered entrenchment maintains essentiality in the evolution of Form I Rubisco complexes

Layered entrenchment maintains essentiality in the evolution of Form I Rubisco complexes

The EMBO Journal - Protein complexes composed of strictly essential subunits are abundant in nature and often arise through the gradual complexification of ancestral precursor proteins....

link.springer.com
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I am pleased to share the peer-reviewed version of our manuscript on in vitro transcription-based biosensors and their application for in vitro pathway prototyping: doi.org/10.1093/synbio/ysae0… Thanks to everyone involved in this work for great teamwork, support and guidance! @erblabs
In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade

In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade

Abstract. In vitro metabolic systems allow the reconstitution of natural and new-to-nature pathways outside of their cellular context and are of increasing

academic.oup.com
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I am very happy to share our pre-print on the prototyping of a complex enzyme cascade (the CETCH cycle) using in vitro transcription-based (ROSALIND) biosensors. Thanks to everybody involved. We are looking forward to your feedback! @erblabs doi.org/10.1101/2024.04.26.5…
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