Anyone on here ever tried using a laser to break open really tough samples for DNA extraction? I'm working on some tough chitinous tissues which nothing seems to reliably lyse them - and thought I might take some inspiration from laser microdissection (which is a tad overkill/$$)
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William Pearman retweeted
Joint consideration of selection and microbial generation count provides unique insights into evolutionary and ecological dynamics ... biorxiv.org/cgi/content/shor… #biorxiv_micrbio
Glad to have this paper out now! If you've ever tried and less-than-successfully sequenced brown algae - polyphenols might be why!
onlinelibrary.wiley.com/doi/…
Looking for some @nanopore help - I have some ~1600 bp amplicons sequenced with the rapid kit (plasmidseq type data) and want to cluster them into ASVs/OTUs - any idea how? Mixed of read lengths due to data type, and only have 2 samples.
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Glad to have this perspective piece out now! We found that when we were trying to develop hypotheses around how microbiomes might change in response to host dispersal - there wasn't much information for non-migratory organisms (e.g., kelp) 1/2
academic.oup.com/femsec/arti…
2/2 - so we wrote up a piece on this, developing models and hypotheses about what we expected would be the case, differences to migratory organisms etc. Hugely grateful to my fantastic co-authors, esp @Grant_Duffy who made the wonderful figure above!
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Glad to have this neat little pre-print out with a bunch of great folks! @NickGreen_chem @Evie_Jensen
biorxiv.org/content/10.1101/…
After struggling so much to get good sequenceable DNA from seaweeds - we did some digging and found some cool results!
To note: this is an EIC from some LC-MS work we did on the DNA. Panel C is the TIC - again showing dry kelp with more 'stuff'.
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Note the 260/230 and 260/280 ratios for these samples were 'good' - and yet seemingly have a bunch of other stuff in there! This was my first time working on this sort of data, so a huge thanks to @NickGreen_chem for being a great guide into the world of chemistry!
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Happy to have this paper out this week! With @Clare_eDNA @CeridwenFraser Fine-scale phylogenetic diversity gradients support the Antarctic geothermal refugia hypothesis | Antarctic Science | Cambridge Core - bit.ly/4bvkHMf
William Pearman retweeted
Excited to share our new paper @EcographyJourna, where we use population genomics to investigate the impact of the 2016 Kaikōura earthquake on Durvillaea #kelp/rimurapa!
First genomic snapshots of recolonising lineages following a devastating earthquake: doi.org/10.1111/ecog.07117
Happy to have this paper from my PhD out this week! Thanks to my fantastic co-authors! @FelixVaux @CeridwenFraser @ProfGemmell enviromicro-journals.onlinel…
William Pearman retweeted
$65m (+/-) was expected to carry past June 30. It is now a cut to be taken as savings? The National Research Priorities process was meant to find a future for large projects but made little sense. So here we are ... defunding climate change, and 10 other important areas. (2/2)
Very pleased to have this paper from my PhD published now! academic.oup.com/aob/advance…
Stoked to be able say I passed my PhD oral examination on Thursday and will be graduating in December! Thanks to my awesome supervisors @CeridwenFraser @ProfGemmell and Sergio (who has made the perhaps wise decision not to join twitter)!
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Super pleased to have submitted my thesis today! A huge thanks to @CeridwenFraser, @ProfGemmell, and Sergio (not on twitter) for supervising me!
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Fantastic talk by @ChrisLDutton on hippo feces loading and community coalescence at #ASLO23
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