William Pearman (@WilliamPearman) profile picture

Research fellow at Auckland Uni. Same handle at the blue place. Evolutionary ecology, microbial ecology. Anglican. He/him

Tāmaki Makaurau, New Zealand
Joined May 2017
Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)Photo by William Pearman (@WilliamPearman)
I have made the move to the other blue place, same name.
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Anyone on here ever tried using a laser to break open really tough samples for DNA extraction? I'm working on some tough chitinous tissues which nothing seems to reliably lyse them - and thought I might take some inspiration from laser microdissection (which is a tad overkill/$$)
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Joint consideration of selection and microbial generation count provides unique insights into evolutionary and ecological dynamics ... biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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Looking for some @nanopore help - I have some ~1600 bp amplicons sequenced with the rapid kit (plasmidseq type data) and want to cluster them into ASVs/OTUs - any idea how? Mixed of read lengths due to data type, and only have 2 samples.
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Glad to have this perspective piece out now! We found that when we were trying to develop hypotheses around how microbiomes might change in response to host dispersal - there wasn't much information for non-migratory organisms (e.g., kelp) 1/2 academic.oup.com/femsec/arti…
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2/2 - so we wrote up a piece on this, developing models and hypotheses about what we expected would be the case, differences to migratory organisms etc. Hugely grateful to my fantastic co-authors, esp @Grant_Duffy who made the wonderful figure above!
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To note: this is an EIC from some LC-MS work we did on the DNA. Panel C is the TIC - again showing dry kelp with more 'stuff'.
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Slight correction: panels D-G are not EICs, rather they are the combined mass spectra between 29 and 30 minutes (the maximum of the peak in panel C). While the boxplot is the distribution of the hypothetical adducts.
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Note the 260/230 and 260/280 ratios for these samples were 'good' - and yet seemingly have a bunch of other stuff in there! This was my first time working on this sort of data, so a huge thanks to @NickGreen_chem for being a great guide into the world of chemistry!
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Happy to have this paper out this week! With @Clare_eDNA @CeridwenFraser Fine-scale phylogenetic diversity gradients support the Antarctic geothermal refugia hypothesis | Antarctic Science | Cambridge Core - bit.ly/4bvkHMf
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William Pearman retweeted
Excited to share our new paper @EcographyJourna, where we use population genomics to investigate the impact of the 2016 Kaikōura earthquake on Durvillaea #kelp/rimurapa! First genomic snapshots of recolonising lineages following a devastating earthquake: doi.org/10.1111/ecog.07117
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$65m (+/-) was expected to carry past June 30. It is now a cut to be taken as savings? The National Research Priorities process was meant to find a future for large projects but made little sense. So here we are ... defunding climate change, and 10 other important areas. (2/2)
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Stoked to be able say I passed my PhD oral examination on Thursday and will be graduating in December! Thanks to my awesome supervisors @CeridwenFraser @ProfGemmell and Sergio (who has made the perhaps wise decision not to join twitter)!
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Super pleased to have submitted my thesis today! A huge thanks to @CeridwenFraser, @ProfGemmell, and Sergio (not on twitter) for supervising me!
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Fantastic talk by @ChrisLDutton on hippo feces loading and community coalescence at #ASLO23
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