A. James Link (@ajlinklab) profile picture

A. James Link

@ajlinklab

Research group at Princeton in Chemical and Biological Engineering

 Princeton, NJ
princeton.edu/~ajlgroup
Joined September 2016
  • Tweets266
  • Following192
  • Followers553
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Photo by A. James Link (@ajlinklab)Photo by A. James Link (@ajlinklab)
A. James Link retweeted
J. Am. Chem. Soc. (@J_A_C_S) profile picture
J. Am. Chem. Soc.@J_A_C_SJun 24
Tangled Tail of Mechanically Interlocked Peptides | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/jac…
Tangled Tail of Mechanically Interlocked Peptides

Tangled Tail of Mechanically Interlocked Peptides

Abstract. First synthesized by chemists in the 1960s, mechanically interlocked molecules (MIMs) were created out of scientific curiosity and thought only t

pubs.acs.org
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A. James Link retweeted
J. Am. Chem. Soc. (@J_A_C_S) profile picture
J. Am. Chem. Soc.@J_A_C_SMar 9
Repositioning the Leader Peptide in Graspetide Biosynthesis | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/jac…
Repositioning the Leader Peptide in Graspetide Biosynthesis

Repositioning the Leader Peptide in Graspetide Biosynthesis

Abstract. Applying enzymes from unique biosynthetic pathways in a combinatorial fashion to garner new-to-nature products has been a long-standing goal of s

pubs.acs.org
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A. James Link retweeted
Toby Johnson (@TobyJohnson144) profile picture
Toby Johnson@TobyJohnson144Mar 9
Excited to share my 1st first name paper from 🐅 @ajlinklab We report the ability to rearrange #RiPP precursor peptides, expanding opportunities for engineering PTMs in vitro and in cellulo including mechanically interlocked peptides 🔗 @LindemannTrust @omenndarlingbio
J. Am. Chem. Soc. (@J_A_C_S) profile pictureJ. Am. Chem. Soc.@J_A_C_SMar 9
Repositioning the Leader Peptide in Graspetide Biosynthesis | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/jac…
Repositioning the Leader Peptide in Graspetide Biosynthesis

Repositioning the Leader Peptide in Graspetide Biosynthesis

Abstract. Applying enzymes from unique biosynthetic pathways in a combinatorial fashion to garner new-to-nature products has been a long-standing goal of s

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New work from @ZhongyueY, @ajlinklab and Yi-Lei Zhao takes a first step toward structural prediction of RiPPs: lasso peptide. nature.com/articles/s41467-0… Access the tool here: lassopred.accre.vanderbilt.e…
Image from Vanderbilt Center for Structural Biology (@VanderbiltCSB)
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Our first step toward structural prediction of RiPPs: lasso peptide. nature.com/articles/s41467-0… This tool can be accessed from here: lassopred.accre.vanderbilt.e… @VanderbiltCSB @VICB_Vanderbilt Great working together with @ajlinklab and Yi-Lei Zhao!
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Things tend to slow down in winter, but Elsa Ding and Yaoyukun Jiang from our group have engineered a 'cold-adapted' version of a mesophilic amylase. Even with a 45-degree temperature drop, we still observe 30% activity. onlinelibrary.wiley.com/doi/… @angew_chem @VanderbiltCSB
Enhancing Cold Adaptation of Bidomain Amylases by High‐Throughput Computational Engineering

Enhancing Cold Adaptation of Bidomain Amylases by High‐Throughput Computational Engineering

This work uncovers molecular determinants of cold adaptation in bidomain amylases by combining MD simulations and biochemical assays, revealing that increased domain separation drives enhanced...

onlinelibrary.wiley.com
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A. James Link retweeted
Yi Tang (@yitang_ucla) profile picture
Yi Tang@yitang_ucla29 Jan 2025
In a year with so little to cheer about so far, I am happy to share our manuscript on a copper-dependent halogenase. Unbelievable effort by GS Yorick Chiang and Scientist @phdmasao at @uclachem @UclaCBE . Thank you to all collaborators and @NIGMS. nature.com/articles/s41586-0…
Copper-dependent halogenase catalyses unactivated C−H bond functionalization

Copper-dependent halogenase catalyses unactivated C−H bond functionalization

Nature - A halogenase enzyme uses the copper in its active site to catalyse iterative chlorinations on multiple unactivated carbon−hydrogen bonds, enabling carbon−hydrogen...

nature.com
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Cysimiditides: RiPPs with a Zn-tetracysteine motif and aspartimidylation biorxiv.org/cgi/content/shor… #biorxiv_biochem
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A. James Link retweeted
Piel Lab (@piel_lab) profile picture
Piel Lab@piel_lab9 Sep 2024
Congrats to @HubrichFlorian & @sanathrajkk0 for this exciting story finally being published. Thanks for the fruitful collaboration with @chekanlab. If you are interested in the chemical space of #RiPP #biosynthetic #enyzmes and #isoprenoids, click here: sciencedirect.com/science/ar…
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Thank you for highlighting our work on the chemoenzymatic synthesis of modified lasso peptides on the covers of @J_A_C_S! 🤩 @Hartrampf_Lab @UZH_ch #peptides
J. Am. Chem. Soc. (@J_A_C_S) profile pictureJ. Am. Chem. Soc.@J_A_C_S1 Jul 2024
From this week's covers: Sidechain- and backbone-modified lasso peptides were produced via a chemoenzymatic approach, offering a promising framework for designing peptide-based drugs. @UZH_Chemistry @NinaHartrampf Learn more ➡️ go.acs.org/a2g
JACS Front Cover Volume 146, Issue 25 Image depicting the article "Merging Flow Synthesis and Enzymatic Maturation to Expand the Chemical Space of Lasso Peptides"

ALT JACS Front Cover Volume 146, Issue 25 Image depicting the article "Merging Flow Synthesis and Enzymatic Maturation to Expand the Chemical Space of Lasso Peptides"

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AlphaFold can't tell you the structure of lasso peptide, a major branch of RiPPs and promising antibiotics. Xingyu wasn't happy about it and decided to create LassoPred. Build your own lasso or download a predicted one: lassopred.accre.vanderbilt.e…. DOI: 10.26434/chemrxiv-2024-q3rn0
Image from Zhongyue (John) Yang (@ZhongyueY)
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A. James Link retweeted
Drew Carson (@DrewCarson712) profile picture
Drew Carson@DrewCarson7123 Jun 2024
Excited to be at @BostonBacteria at Boston Univ! Looking forward to some great science #BBM2024 🧫 I’m presenting poster number 104 tomorrow, come say hi!
Image from Drew Carson (@DrewCarson712)
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Our chemoenzymatic synthesis of the lasso peptide Microcin J25 is out now in @J_A_C_S! We used flow-SPPS to synthesize several sidechain- and backbone-modified precursor peptides and demonstrated their in vitro transformation. pubs.acs.org/doi/10.1021/jac… @UZH_Chemistry @Hartrampf_Lab
Merging Flow Synthesis and Enzymatic Maturation to Expand the Chemical Space of Lasso Peptides

Merging Flow Synthesis and Enzymatic Maturation to Expand the Chemical Space of Lasso Peptides

Abstract. Many peptidic natural products, such as lasso peptides, cyclic peptides, and cyclotides, are conformationally constrained and show biological sta

pubs.acs.org
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A. James Link retweeted
Yi Tang (@yitang_ucla) profile picture
Yi Tang@yitang_ucla15 May 2024
Early Announcement! simbhq.org/np/ Together with Brad @BradMoore_SIO, Jaclyn @JMWinterlab, and Alessandra @EustaquioLab, We look forward to welcoming you to the 2025 @SIMB_Microbio Natural Product meeting at San Diego! Keynote speaker Prof. Jennifer Doudna! @doudna_lab
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A. James Link retweeted
J. Am. Chem. Soc. (@J_A_C_S) profile picture
J. Am. Chem. Soc.@J_A_C_S20 Apr 2024
Cyclic Peptides from Graspetide Biosynthesis and Native Chemical Ligation | Journal of the American Chemical Society @Princeton @EPrinceton @PrincetonChem #Peptides #Cyclic #Graspeptide #Biosynthesis #NativeChemical #Ligation pubs.acs.org/doi/10.1021/jac…
Cyclic Peptides from Graspetide Biosynthesis and Native Chemical Ligation

Cyclic Peptides from Graspetide Biosynthesis and Native Chemical Ligation

Abstract. The ribosomally synthesized and post-translationally modified peptide (RiPP) superfamily of natural products includes many examples of cyclic pep

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A. James Link retweeted
Top Science (@isciverse) profile picture
Top Science
@isciverse18 Apr 2024
Cyclic Peptides from Graspetide Biosynthesis and Native Chemical Ligation @J_A_C_S #Chemistry #Chemed #Science #TechnologyNews #news #technology #AcademicTwitter #AcademicChatter pubs.acs.org/doi/10.1021/jac…
Image from Top Science (@isciverse)
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A. James Link retweeted
ASBMB (@ASBMB) profile picture
ASBMB@ASBMB26 Mar 2024
#DiscoverBMB: Join us for the #biochemistry and #biotechnology of natural product #genome mining session at 9 a.m. in 214BC! Learn about how #MotherNature’s molecules can be used to create novel solutions. Details: guidebook.com/g/#/guides/dis…
Image from ASBMB (@ASBMB)
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A. James Link retweeted
Drew Carson (@DrewCarson712) profile picture
Drew Carson@DrewCarson71225 Mar 2024
Our new paper is just out now in ACS Chemical Biology! Here we uncover the transport pathway that the antimicrobial lasso peptide cloacaenodin uses to be able to kill susceptible bacteria. This helps explain the narrow-spectrum activity we observe. pubs.acs.org/doi/10.1021/acs…
Antimicrobial Lasso Peptide Cloacaenodin Utilizes a Unique TonB-Dependent Transporter to Access...

Antimicrobial Lasso Peptide Cloacaenodin Utilizes a Unique TonB-Dependent Transporter to Access...

Abstract. The development of new antimicrobial agents effective against Gram-negative bacteria remains a major challenge in drug discovery. The lasso pepti

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A. James Link retweeted
Yi Tang (@yitang_ucla) profile picture
Yi Tang@yitang_ucla15 Feb 2024
What is cycloleucine?! Read our preprint to see what it is and how nature makes it! chemrxiv.org/engage/chemrxiv… Led by @phdmasao, Collaborated with @TheNelsonLab

Discovery and Characterization of Pyridoxal 5’-Phosphate-Dependent Cycloleucine Synthases | ChemRxiv

Pyridoxal 5’-phosphate (PLP)-dependent enzymes are the most versatile biocatalysts for synthesizing non proteinogenic amino acids. a,a-disubstituted quaternary amino acids, such as 1-amino-1-cyclop...

chemrxiv.org
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A. James Link retweeted
Ania Fryszkowska (@fryania) profile picture
Ania Fryszkowska@fryania2 Feb 2024
Engineering Hydroxylase Activity, Selectivity, and Stability for a Scalable Concise Synthesis of a Key Intermediate to Belzutifan - Cheung-Lee - Angewandte Chemie International Edition - Wiley Online Library onlinelibrary.wiley.com/doi/…
Engineering Hydroxylase Activity, Selectivity, and Stability for a Scalable Concise Synthesis of a...

Engineering Hydroxylase Activity, Selectivity, and Stability for a Scalable Concise Synthesis of a...

Alpha-ketoglutarate-dependent dioxygenases (αKGDs) are promising biocatalysts for selective oxidations, but their practical use is limited by substrate scope and stability. We engineered an αKGD to...

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