Forrest Lefler (@flefler_) header
Forrest Lefler (@flefler_) profile picture

Forrest Lefler

@flefler_

Cyanobacterial treatment, ecology, taxonomy and systematics, and (meta)genomics. CyanoSeq news/updates will be posted here.

github.com/flefler/CyanoSeq
Joined April 2022
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WE ARE STANLEY CUP CHAMPIONS!!!!
Red gradient background. In the center, there are the outlines of several silver shields. At the very top, is the bowl of the Stanley Cup. Below that, working left to right, are Aleksander Barkov, Sergei Bobrovsky, and Matthew Tkachuk on the first line. On the second line are Anton Lundell, Sam Reinhart, Gustav Forsling, Sam Bennett, and Eetu Luostarinen. On the third line are Ryan Lomberg, Kevin Stenlund, Dmitry Kulikov, Niko Mikkola, Brandon Montour, Oliver Ekman-Larsson, and Kyle Okposo. On the fourth line are Tobias Bjornfot, Uvis Balinskis, Anthony Stolarz, Josh Mahura, Steven Lorentz, Nick Cousins, Jonah Gadjovich, and Spencer Night. In the center is the 2024 Stanley Cup logo and below that it says: “Stanley Cup Champions” with the Panthers shield directly below. At the bottom of the graphic, in celebratory cutouts, are Carter Verhaeghe, Vladimir Tarasenko, Evan Rodrigues, and Aaron Ekblad.

ALT Red gradient background. In the center, there are the outlines of several silver shields. At the very top, is the bowl of the Stanley Cup. Below that, working left to right, are Aleksander Barkov, Sergei Bobrovsky, and Matthew Tkachuk on the first line. On the second line are Anton Lundell, Sam Reinhart, Gustav Forsling, Sam Bennett, and Eetu Luostarinen. On the third line are Ryan Lomberg, Kevin Stenlund, Dmitry Kulikov, Niko Mikkola, Brandon Montour, Oliver Ekman-Larsson, and Kyle Okposo. On the fourth line are Tobias Bjornfot, Uvis Balinskis, Anthony Stolarz, Josh Mahura, Steven Lorentz, Nick Cousins, Jonah Gadjovich, and Spencer Night. In the center is the 2024 Stanley Cup logo and below that it says: “Stanley Cup Champions” with the Panthers shield directly below. At the bottom of the graphic, in celebratory cutouts, are Carter Verhaeghe, Vladimir Tarasenko, Evan Rodrigues, and Aaron Ekblad.

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Bro please come to the conference. Registration fee $1000 ($950 early bird), flights & accommodation $3000. You'll present for 6 minutes and get roasted. You can't present virtually because...look you just can't ok. There's a yoga session. You'll get a point on your CV bro please
Image from Daniel Gould, MD, PhD (@DJGould94)
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Forrest Lefler retweeted
Anders Kiledal (@eandersk) profile picture
Anders Kiledal@eandersk29 Jan 2024
Want to determine Microcystis diversity in your samples? We evaluated all Microcystis core genes to identify the best markers and developed optimal primers for sequencing. Markers also predictive of gene content, including for bioactive compound synthesis. doi.org/10.1016/j.hal.2024.1…
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Forrest Lefler retweeted
Audrey Goeckner (@PondGator) profile picture
Audrey Goeckner@PondGator27 Apr 2023
Replying to @flefler_
@flefler_ & I ran trial L/D net N2 incubations to see if this picocyanobacteria, Vulcanococcus sp., is fixing N! We cultured some in N-free media & N-enriched media for a month (pic1), & diluted each into either N-free media or N-enriched media (pic2) for the incubation (pic3)
Image from Audrey Goeckner (@PondGator)
Image from Audrey Goeckner (@PondGator)
Image from Audrey Goeckner (@PondGator)
Image from Audrey Goeckner (@PondGator)
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#CyanoSeq v1.2 is now available! zenodo.org/record/7864137
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CyanoSeq is a curated database of #cyanobacteria 16S rRNA sequences for taxonomic assignment of metabarcoding reads, and can also be used for #cyano systematics etc
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Our (with @algae_slayer and @vivalgae) manuscript was recently accepted in @JPhycology here doi.org/10.1111/jpy.13335
Cyanoseq: A database of cyanobacterial 16S rRNA gene sequences with curated taxonomy

Cyanoseq: A database of cyanobacterial 16S rRNA gene sequences with curated taxonomy

Cyanobacteria are photosynthetic bacteria that occupy various habitats across the globe, playing critical roles in many of Earth's biogeochemical cycles both in both aquatic and terrestrial systems....

onlinelibrary.wiley.com
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