CRISPR is a magical tool for modern biology.
Egypt
Joined September 2011
- Tweets57
- Following1.1K
- Followers138
- Likes318
Karam MOSTAFA retweeted
Expanding the Plant Genome Editing Toolkit! 🍅⚡ Jian-Kang Zhu & Zhaobo Lang's Teams Establish High-Efficiency Cas12i3 Editing in Tomato in JIPB (2026)!
Quintuple mutant Cas-SF01 achieves up to 90.2% editing efficiency at 5′-TTN-3′ PAMs, matching Cas9 while boosting fruit GABA levels! 🎯📦
Key Technological Breakthroughs:
🧫 Hairy Root Rapid Screening: Accelerated variant & crRNA validation via A. rhizogenes hairy roots within weeks prior to stable transformation.
🧬 Cas-SF01 Quintuple Engine: Engineered S7R/D233R/D267R/N369R/S433R mutant outpaced single/double variants across all target loci.
📈 Up to 90.2% Editing Efficiency: Reached 87.8%–90.2% in SlTDC1 and 81.9%–89.6% in SlSGR1, rivaling Cas9 performance.
🍅 Metabolic Trait Improvement: Targeted disruption of the SlGAD3 CaMBD domain enriched GABA content in edited T0/T1 fruits with zero off-target mutations.
A major leap for TTN PAM genome engineering in dicot crops! 🚀🌟
doi.org/10.1111/jipb.70391
#TomatoGenomics #Cas12i3 #JIPB2026 #CRISPR #PlantBiotech #GABA #SUSTech
🌱🍅One more article has been published in @journal_of_Crop_Health.
Our findings provide valuable insights into the role of the SlFLA1 gene in response to biotic stress caused by bacterial and nematode infections.
@musakavas78
link.springer.com/article/10…
🪴 Our latest article has been published in @Journal of Plant Growth Regulation. 💡Our findings highlight the roles of HtXTH7 and HtXTH15 in tomato responses to heat and drought stress. @musakavas78
link.springer.com/article/10…
Karam MOSTAFA retweeted
Genome degradation in plant tissue culture | PNAS pnas.org/doi/10.1073/pnas.25…
🪴 The first chapter of my PhD research has been published in the @PlantCellRep journal.
☘️Keep your time and evaluating the efficiency of your gRNA within one month using cost-effective methods, based on rhizogenes in tomato. @musakavas78
link.springer.com/article/10…
Our new review article “Exploring Microbial Approaches and CRISPR-based Strategies for Enhancing Plant Resilience under Abiotic Stress” has been published in @PPLplantarum.
Special thanks to @Recrop22157 for their support.
@musakavas78
onlinelibrary.wiley.com/doi/…
Our latest article has been published, have a look.
💡This review article offers an integrated perspective on NHX-mediated pathways and regulation. It outlines practical approaches for implementing NHX-informed strategies to develop salt-tolerant crops and promote sustainable agriculture in the context of climate change.
@musakavas78 @velimirmladenov @Recrop22157
sciencedirect.com/science/ar…
Karam MOSTAFA retweeted
Each month we will be featuring one of our authors in @AABiologists new webinar series 🌱
Earlier this week "All aabout CRISPR" featured @krushxp90 talking about his recent review: doi.org/10.1111/aab.70035
ICYMI, you can watch it here: buff.ly/e8xPNFg
Karam MOSTAFA retweeted
Hello September!!
Our latest newsletter is out now 😍
Grab a cuppa and read it here👉 buff.ly/utS54vb
I am delighted that my meeting report has been published in the @AABiologists September newsletter!
Karam MOSTAFA retweeted
🌱 Announcing our brand new webinar series all 'aabout' #plantscience & #sustainableagriculture
Starting 23 Sept with talks on #crop #CRISPR from:
🌾@SadiyeHayta
🍓 #ECR Ryan Creeth
🥔@AnnalsApplBio author @krushxp90
Register for free: buff.ly/qdflQbB
Karam MOSTAFA retweeted
Had a great week sharing our experiences on plant transformation & gene editing at the 1st AAB @AABiologists PlantEd Training School, HHU Düsseldorf. Expertly organised by @GoeHensel, with great lectures from @SadiyeHayta, @MarkSmedley15 & Katrijn Van Laere @ILVOvlaanderen
It was a pleasure to participate in this training.
Day 2 of the AAB-PlantEd Training School and it was into the lab for our trainees!
#plantscience #earlycareer
🌾 Have a look at our current research that was published in Plant Physiology and Biochemistry Journal
📖 Synergistic effect of biochar along with gypsum and foliar nutrient strategies on the growth, yield, metabolism, and gene expression in barley plants under salinity stress.
🔗 lnkd.in/dsBcqFqm
🔬 A new review article
📖 CRISPR vs. nematodes: A new era of genetic solutions for potato crop protection
🥔 In this review, we discussed the potential of CRISPR to address nematodes, and the current limitations associated with its application in potato.
onlinelibrary.wiley.com/doi/…
Karam MOSTAFA retweeted
Join us in celebrating the remarkable achievement of our MSc and PhD Food Science graduates as they are conferred with their degrees today!
Your dedication and hard work have truly paid off. Here's to a bright future filled with endless opportunities!
@UCC @SEFSUCC @akellyucc
Karam MOSTAFA retweeted
Article Alert! 📢
Genome-wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses
acsess.onlinelibrary.wiley.c…
#OpenAccess
Karam MOSTAFA retweeted
#GenomeEdited #NonBrowning_Potato is close to release in Argentina.
Agricultural Technology - INTA of Argentina is close to releasing the first genetically edited potato in Latin America.
#CRISPR agrobio.org/noticias/papa-ed…
