ALT The gavage/fomite-treated mice are significantly protected against L. monocytogenes infection. (A) Scheme of the infection. SPF and pet store mice (PS) were used as controls. Created with BioRender.com. (B) Survival rate after L. monocytogenes infection. Significances of survival were determined by the log-rank (Mantel–Cox) test. (C) Body weight changes after L. monocytogenes infection. Statistical significance was determined by a mixed-effects analysis with Tukey’s multiple comparison test. Asterisks indicate significant differences between SPF mice and either GaF or pet store mice. (D) Bacterial load in the liver on day 4 after the challenge. Bacterial load was normalized by the weight of the liver. Significances were determined by the one-way ANOVA test with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
ALT D3 and 2C3 VHHs used as 89Zr-labeled immuno-PET/CT agents detect 2D2 CD4+ T cells in 2D2tg mice. (A) Schematic of site-specific modification of the VHH with DFO and PEG20. (B) SDS-PAGE analysis of purified and PEG20-DFO modified versions of the indicated VHHs. (C) Experimental outline. (D) D3, (E) 2C3, and (F) H4 VHH imaging of 2D2tg mice (top, n = 2) and C57BL/6 mice (bottom, n = 1) at the indicated time points. Green arrowheads indicate cervical, axillary, and inguinal lymph nodes. (G) Ex vivo biodistribution of 89Zr-labeled D3, 2C3, and H4 VHH at the 72-hour timepoint. Data represent one experiment performed in female mice (9 weeks old).
ALT Smiling NHS nurse in blue uniform encouraging flu vaccination with message Stay Strong. Get Vaccinated.
ALT Cell population alterations in the gingiva and systemic tissues in TET2-driven CHIP. Lethally irradiated CD45.1 mice were transplanted with either 10% Tet2–/– CD45.2 BM cells and 90% WT CD45.1 BM cells (“10% Tet2–/– BMT” group) or with 10% Tet2+/+ CD45.2 BM cells and 90% WT CD45.1 cells (“10% Tet2+/+ BMT” group).
