Fluorescence Microscopy, lead developer of the https://nitter.cf/t.co/L6tChEyj9U project for Structured Illumination. Bielefeld University. Work here, more @LightAndScience
Louvain, Belgium
Joined April 2016
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Blue-print for an SLM-based SIM system, made (almost) completely from readily available components:
doi.org/10.1364/BOE.423492
By Robin Van den Eynde at @DedeckerLab
Marcel Müller retweeted
Doing multicolor (single-molecule) imaging? Tired of waiting for each color?
Want simultaneous imaging with full field of view but without the multicamera hassle?
Take a look at our 'Circulator'! 🔥💪
t.ly/lHZq. Very excited so please RT! (1 of 5)
#microscopy
Marcel Müller retweeted
Applications are now open for our advanced #SIM practical course! If you are an #imagingscientist working in a #corefacility or research group supporting or soon to be supporting SIM projects, then this is the course for you! More info here micronoxford.com/sim-practic… 🔬
Marcel Müller retweeted
After a long programming week we released a new @napari_imaging plugin for #StructuredIlluminationMicroscopy, #gpu accelerated with #PyTorch. Drop your raw images in #napari and let us know if you manage to reconstruct your #SuperResolution images.
napari-hub.org/plugins/napar…
ALT cross-correlation of the raw data to locate the carrier frequencies
Our third (and for now last...) paper on DMD-SIM, where we use temperature control of a diode laser to tweak its wavelength to match the DMD's blazed grating.
doi.org/10.1364/OE.437822
It is an alternative to the very nice approach of P. Brown et al. (@DougPShepherd, @ptbrown1729), where they use a voice coil mirror to slightly adjust the angle instead of shifting the laser wavelength:
osapublishing.org/boe/fullte…
Together with the setup's blue-prints by @NanoSand_Alice
pubs.acs.org/doi/abs/10.1021…
and the simulation framework
royalsocietypublishing.org/d…
there's now quite a bit of detail on how to use DMDs in SIM applications.
Marcel Müller retweeted
So happy to see this paper out! 😊Together with @SHugelier @mueller_physics, @Sam_Duwe and @DedeckerLab we present a simple method to efficiently separate spectrally overlapping FPs! 🔬 rb.gy/5r7izp
Marcel Müller retweeted
heise+ | UC2-Modulbaukasten: Optik‑ und Mikroskopie-System aus dem 3D-Drucker heise.de/ratgeber/UC2-Modulb… #heiseplus #Baukasten
Marcel Müller retweeted
Pleasure to see paper of @Robin_VdE et co-authors as the top download in Biomedical Optics Express! 🥳
Find full paper: osapublishing.org/boe/fullte…
and SI here:
zenodo.org/record/4750228#.Y…
Marcel Müller retweeted
To all SIM-people: ever wondered about noise artifacts in SIM reconstructions and about what's real and what's not? We developed reconstruction algorithms that handle the noise artifacts (2D & 3D). No more SIM fudging! Check out: nature.com/articles/s41592-0…
Online today, including our deep dive into simulating digital micromirror devices for use in SIM.
Paper: royalsocietypublishing.org/d…
Full issue: royalsocietypublishing.org/t…
The Philosophical Transactions A (@RSocPublishing) issue on 'Super-resolution structured illumination microscopy (Part 1)' will be online on the 26th of April.
Here's the list of articles in the 1st volume.
@HeintzmannLab @beniroquai @StefanieReiche6 @LSchermelleh
#SIMposium
For those using IDS uEye cameras in MicroManager, I finally got a chance to compile our updated device adapter for the current MicroManager nightly:
github.com/biophotonics-biel…
Didn't get a chance for any extensive testing yet, but on first glance, it works as well as before.
Encoding next weeks lecture.... We've decided to prerecord lectures and just have a weekly Q&A session, to give people some more flexibility. Also, eventually optional practicals in small groups, and others can join via video call. Quite a bit of effort, but hopefully worth it.
Deep learning to denoise SIM data, with two approaches, (a) having the network learn the complete reconstruction and (b) only using the network for denoising and artifact reduction after "classic" reconstruction.
Deep-learning based denoising and reconstruction of super-resolution structured illumination microscopy images biorxiv.org/cgi/content/shor… #bioRxiv
From my side, this started some years ago, as an idea for a "PhD project" design contest.
nitter.cf/mueller_physics/status…
Deep neural networks for SMLM: arxiv.org/abs/1801.09631
Anyone interested in / working on trying this for (deconvolution-based) SIM reconstructions? We've thrown some ideas around in our 'PhD projects design contest'. And hey, we'd have a logo... #NeuralNetworks #fairSIM
Our simulation approaches to use DMDs with coherent light to build SIM microscopes. Complements the cost-effective DMD SIM that we were able to build based on some of these calculations.
Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy biorxiv.org/cgi/content/shor… #bioRxiv
Marcel Müller retweeted
Today I highlight the physicist Alice Sandmeyer @NanoSand_Alice 🔬👩🏻💻 for my DPG #physikerinderwoche initiative - Check out her profile and read her & @mueller_physics’s paper about low-cost & high speed SIM biorxiv.org/content/10.1101/… – at Lansdowne, VA
Alice Sandmeyer #PhysikerinderWoche - @NanoSand_Alice focused her PhD research on designing compact & cost-efficient fluorescence microscopes... @komm_mach_mint @BMBF_Bund @ResearchGermany @unibielefeld #physikerin #physikerinnen dpg-physik.de/vereinigungen/…
Marcel Müller retweeted
Today @HHMIJanelia’s Optical Interest Group hosted @mueller_physics who presented the current state of the #fairSIM project. Not only did he highlight his @FijiSc fairSIM plugin, but also his team’s efforts to improve SIM scopes on multiple fronts... Just impressive! #openscience – at Lansdowne, VA
