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Happy to share the latest paper from my postdoc in @davidrliu’s group, out now in @NatureNano! We develop a workflow for optimizing in vivo prime editing delivered via LNPs, advancing our ability to benefit patients with prime editing. See David’s thread below!
Today in @NatureNano, we report an all-RNA lipid nanoparticle (LNP) system for efficient in vivo prime editing (PE). We identify bottlenecks in transient prime editor delivery, develop a workflow for LNP optimization, and use it to rescue a mouse model of phenylketonuria.
drive.google.com/file/d/1-rP…
1/12
Allen Jiang retweeted
News & Views:
A non-viral path to efficient and safe prime editing in vivo
"Solving four delivery bottlenecks enables #lipid #nanoparticles to achieve curative #primeediting in mice."
nature.com/articles/s41565-0…
Huge thanks to my talented co-lead on this project
@Ana_Cristian_, folks in the the @liugroup (@paulchenz for the awesome cryo-EM images + analysis!), @kiranmusunuru and Xiao Wang's groups at @PennMedicine, and @WangXiaoLab
at the @broadinstitute!
This work complements recent Liu lab efforts on AI-guided prime editor redesign (nature.com/articles/s41587-0…) and new epegRNA motifs incorporated into our platform (nature.com/articles/s41587-0…).
Excited to share the first co-first author paper from my postdoc in @davidrliu’s group @broadinstitute, out now in @NatureBiotech! We used AI-guided computational redesign to develop new PE8 prime editors. See David’s thread below!
Today in @NatureBiotech we report a new suit of PE8 prime editor proteins. PE8 variants were developed from laboratory-evolved PE6 proteins using AI-guided protein redesign. This approach combines recent advances in computational protein design and directed evolution to increase prime editing efficiency, especially in transient therapeutically relevant delivery settings such as mRNA+pegRNA electroporation into primary cells, eVLP delivery of prime editing RNPs, and LNP-mediated mRNA+pegRNA delivery in mice.
drive.google.com/file/d/13VT…
1/11
This project was an awesome collaboration with @Allentaoyz, @HoltSakai, and @NicholasKrasnow and many others in the Liu Group. Stay tuned for our dedicated story on an optimization workflow for in vivo prime-editing LNPs!
To unlock the full clinical potential of highly stable editors like PE8, we need advanced delivery tech. I'm excited to tackle this by engineering non-viral delivery systems in my independent lab at @UclaCBE starting July 2027! Recruiting for Fall '27: sites.google.com/g.ucla.edu/…
New paper in @NatureBiotech! Congrats to @HoltSakai, @Sarah_E_Pierce and rest of the team in the @liugroup. Glad to have contributed to the LNP experiments where we demonstrate improvements to in vivo prime editing with new epegRNA motifs. Stay tuned for more work from the group!
Today in @NatBiotech, we report the directed evolution of structured RNA motifs that enhance the efficiency of prime editing. Iterated high-throughput pooled screens and mutagenesis of these small RNA elements improved transient pegRNA lifetime.
drive.google.com/file/d/1Nyt…
1/11
Allen Jiang retweeted
Today in @natBME we report an in vivo base editing strategy that corrects a common disease-causing mutation and rescues pathology in a mouse model of Zellweger spectrum disorder (ZSD) and restore peroxisomal function in patient derived cells. This work highlights the promise of precision genome editing for treating peroxisomal biogenesis disorders.
1/13
Allen Jiang retweeted
Today in @CellCellPress we report the use of prime editing to correct several mutations that cause alternating hemiplegia of childhood (AHC), a rare and devastating neurodevelopmental disorder, in patient-derived cells and in two mouse models.
drive.google.com/file/d/1ibC…
1/10
Allen Jiang retweeted
Breaking News: A baby with a rare disorder made medical history by receiving the first custom gene-editing treatment. The technique used has the potential to help people with thousands of other uncommon genetic diseases. nyti.ms/4j3xGs8
I'm excited to share that I will be joining @UclaCBE as an Assistant Professor starting July 2027!
I'm grateful to the many amazing mentors, colleagues, and collaborators that have supported me on this journey, including my PhD advisors Bob Langer and Dan Anderson and my postdoc advisor @davidrliu
Allen Jiang retweeted
Happy to share our latest manuscript published in Nature Protocols @NatureProtocols @NaturePortfolio named: “mRNA lipid nanoparticle formulation, characterization and evaluation”! @ma_yutian @rachel_vkm @UNC @UNCPharmacy @DPMP_UNC
Manuscript link here: rdcu.be/ec3lX
Excited to share the last piece of my PhD thesis published today in @J_A_C_S where we engineer zwitterionic polymer modified LNPs to replace PEG and stabilize LNPs during nebulization, enabling efficient inhaled mRNA delivery: pubs.acs.org/doi/10.1021/jac…
Huge thanks to co-author @Dr_S_Lathwal, former undergrad Sabrina Meng, and all the other folks in the Langer and Anderson labs for this awesome collaboration! @MITChemE @kochinstitute
Allen Jiang retweeted
Scientists have developed a prime editing approach that efficiently corrects the most common cystic fibrosis mutation in human lung cells, which could pave the way to a one-time treatment as effective as existing daily therapies. broad.io/CF-PrimeEditing-New…
ALT Illustration of a woman with an overlay of her lungs, showing lightning bolts indicating pain or discomfort. Beside her is a DNA double helix with colored segments, connected to a chart with matching colors. This represents the genetic aspect of cystic fibrosis and its impact on the respiratory system.
Allen Jiang retweeted
Excited to share our latest research published in @NatureMaterials nature.com/articles/s41563-0… It showcases an innovative approach combining machine learning with advanced combinatorial chemistry to enhance the discovery of ionizable lipids that are crucial for mRNA delivery.
Honored to have received the Controlled Release Society's PhD thesis award! This award wouldn't have been possible without the support of my PhD advisors Bob Langer and Dan Anderson @MITChemE @kochinstitute
controlledreleasesociety.org…
