Graduate Researcher with the Slavov Lab
Joined September 2018
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Head over to MaxQuant.live to try out the newest version of @TheCoxLab and @quiztoph's excellent software platform for targeted and prioritized data acquisition!
It was a pleasure collaborating with you guys, as well, @quiztoph!
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Gray Huffman retweeted
We always aimed to link single-cell protein measurements to cellular functions and regulatory mechanisms.
This project was a major step for us in this directions, especially with the direct measurements proteolysis products: biorxiv.org/content/10.1101/… Data: scp.slavovlab.net/Huffman_et…
Gray Huffman retweeted
Did you think that data completeness of DIA could be improved?
plexDIA increases the consistency of protein quantification, resulting in over 2-fold reduction of missing data across samples.
biorxiv.org/content/10.1101/…
Gray Huffman retweeted
I'm very excited to share the work we've done @slavovLab in collaboration with @DemichevLab! Increasing the throughput of sensitive proteomics by plexDIA
biorxiv.org/content/10.1101/…
Gray Huffman retweeted
Yes, stay tuned ... more is coming, ... and it cannot be soon enough.
The future of DIA proteomics is multiplexed. Fantastic collaboration with @slavovLab, finally public! biorxiv.org/content/10.1101/…. For now we demonstrate fold-change throughput boost, other applications coming soon.
Gray Huffman retweeted
.@_lukekhoury and I highlight the identification of a signaling interactome and outline how MS proteomics can be combined with deep learning towards comprehensive identification of regulatory protein networks pubs.acs.org/doi/10.1021/acs…
Interested in evaluating your own single-cell proteomics data sets with a user friendly and well documented pipeline?
Check out the scp R package and accompanying preprint from @c_vanderaa and @lgatt0:
biorxiv.org/content/10.1101/…
#SingleCell #Bioinformatics #proteomics
Gray Huffman retweeted
Led by @harrisonspecht and just in time for #scp2019, our next-generation version of single cell proteomics: SCoPE2 is now out on bioRxiv! In it we apply SCoPE2 to the study of macrophage differentiation. nitter.cf/biorxivpreprint/status…
High-throughput single-cell proteomics quantifies the emergence of macrophage heterogeneity biorxiv.org/cgi/content/shor… #bioRxiv
Gray Huffman retweeted
Thanks for coming to my twitter poster! Development of click-amplifying FISH (clampFISH) probes to exponentially amplify RNA fluorescent signal in situ with single molecule resolution. All questions and comments welcome! @arjunrajlab #RSCPoster #RSCChemBio
If you're interested in optimizing your LC-MS/MS-driven #proteomics workflows, we hope you'll check out DO-MS, an opensource R-based dashboard for visualizing search results #RSCPoster #RSCChemBio #RSCAnalytical
...whereas cross-cell-type peptide abundances are not.
This type of plot can serve as a useful metric for determining the accuracy of quantitation, as well as for determining potential sample prep issues, such as poor label quenching (for which figure a. can also be useful).
