Life sciences, structural biology, ribosome
RNA Therapeutics, UMassMed
Joined August 2020
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All life depends on accurate translation (both intracellular & international). Using time-resolved cryo-EM, we watched ribosomes decode mRNA, aided by EF-Tu and GTP. See our animations & article (nature.com/articles/s41586-0…) on how ribosomes proofread tRNAs to make correct proteins:
Thank you Israel.
Spectacular stuff from @KorostelevLab and Niko Grigorieff lab.
Identifying new kind-of-initiation factors in single particle data from lamellae of cells... technically very clever and with high biological relevance.
Congrats!
Looking forward to reading it in detail.
We find that 5MP proteins promote high-fidelity initiation by binding 48S scanning complex to bypass weak start codons. We propose renaming them eIF1S (euk initiation factor 1 scanning).
Great collab w/ @grigoriefflab.bsky.social
Congrats to all authors! science.org/doi/10.1126/scia…
Korostelev Lab retweeted
The Gonzalez Lab at Columbia University is seeking a scientific leader!
Join us in a Research Scientist position, driving single-molecule, structural, and biochemical studies of translation, translational control, and other RNA-based processes.
Apply: apply.interfolio.com/176949
GTPBP1 is a GTPase implicated in neurodevelopment. Our cryo-EM maps show GTPBP1 delivering tRNA to ribosome. GTPBP1 promotes more stringent mRNA decoding than the bona fide delivery factor eEF1A.
Congrats to Denis Susorov and collab with Pestova lab! tinyurl.com/3smdvt3v
Using "in extracto cryo-EM", we visualize ribosomes in mammalian lysates, including RRL. "Hibernating" ribosomes carry proteins that protect functional centers. These include elongation factor eEF2, LARP1 implicated in mTOR signaling, eIF5A etc. biorxiv.org/content/10.1101/…
Korostelev Lab retweeted
Preprint from our collaboration with the labs of Gerry Wright and Shura Mankin @shuramankin identifying the first translation inhibitor binding to the E-site of the bacterial ribosome… researchsquare.com/article/r…
ROOL monomers entangle with other monomers via strand swapping to form octameric nanocages.
nature.com/articles/s41586-0…
With our RTI colleagues Xiaobin Ling and @WenwenFang2, we report cryo-EM structures of enigmatic bacterial RNA nanocages made of long-noncoding ROOL RNAs. Octamers are held by "A-minor staples" and other interactions. Congrats Xiaobin, Dima et al!
nature.com/articles/s41586-0…
Activation of Retron-Septu for antiphage defense. Congrats @futianmin and all co-authors!
science.org/doi/full/10.1126…
Korostelev Lab retweeted
“Undermining the future of American science is not a path to accountability — it’s a path to decline.”
In a powerful new piece, my colleague Daphna Shohamy explains how canceled grants are driving young scientists out of science—and out of the country.
🔗 statnews.com/2025/05/07/us-s…
Korostelev Lab retweeted
In our newly published study we show that the proline-rich antimicrobial peptide (PrAMP) Bac5 has a distinct mode of interaction with the ribosome compared to other PrAMPs…academic.oup.com/nar/article…
Korostelev Lab retweeted
We developed a new siRNA drug candidate for prion disease, a @broadinstitute / @RTI_UMassChan collaboration. We now have an open IND meaning FDA has given us permission to start first-in-human trials. Work remains before trials can launch. The full update: cureffi.org/2025/04/14/open-…
Korostelev Lab retweeted
I’m happy to share our recent manuscript in which we are structurally and biochemically investigating 3'-5' tRNA splicing ligases, including a #cryoEM structure of the five-subunit D. rerio tRNA ligase complex.
jbc.org/article/S0021-9258(2…
Korostelev Lab retweeted
Fresh off the press…our latest study on the Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit | great collaboration with @inada_lab and Zaher lab. Data collected at @DCI_EM.
PNAS pnas.org/doi/10.1073/pnas.24…
Korostelev Lab retweeted
Anastasia Khvorova, PhD, named 2025 Else Kröner Fresenius Prize for Medical Research awardee for her pioneering work in the field of RNA-based therapies: direc.to/nacN
#EKFprize25 #EKFS #ElseKrönerFreseniusStiftung #RNA #RNAtherapies @EKFStiftung @RTI_UMassChan
Korostelev Lab retweeted
Biological systems find ingenuous solutions. Almost anything that can happen, happens.
Still, I would not have guessed a role of RNA-binding proteins on the cell surface.
The Flynn lab are boldly investigating glycoRNAs and following their leads.
Korostelev Lab retweeted
Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation
cell.com/cell/fulltext/S0092…
Korostelev Lab retweeted
Our review on small endogenous RNAs in animals is online. We thank @MartinjSimard, @SvobodaLab and the third, anonymous, reviewer for helping us improve our manuscript. bit.ly/3Ebk931
Gabriel Demo and colleagues report how a small archaeal protein (aRDF) dimerizes 30S subunits to prevent ribosome formation:
academic.oup.com/nar/article…
Korostelev Lab retweeted
Using reporters with long unstructured 5’ UTRs, we examined which initiation factors are limiting for 40S scanning. Our results&literature led us to the model that 40S scans 5’ UTRs by one-dimensional diffusion rather than helicase-driven translocation. biorxiv.org/content/10.1101/…
