Robert Battaglia (@RNABob) header
Robert Battaglia (@RNABob) profile picture

Robert Battaglia

@RNABob

Postdoc Li lab @ MIT. PhD Ke lab @ Cornell. Studying biology.🧬🧫

Joined April 2019
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  • Following117
  • Followers55
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Robert Battaglia retweeted
Gene-Wei Li Lab (@GeneWeiLiLab) profile picture
Gene-Wei Li Lab@GeneWeiLiLab10 Jan 2024
We recently found an unexpected deletion in the amyE-back homology arm commonly used for cloning in B. subtilis -- check out the details in our short preprint: biorxiv.org/content/10.1101/…
A historical sequence deletion in a commonly used Bacillus subtilis chromosome integration vector...

A historical sequence deletion in a commonly used Bacillus subtilis chromosome integration vector...

Since the 1980s, chromosome-integration vectors have been used as a core method of engineering Bacillus subtilis . One of the most frequently used vector backbones contains chromosomally derived...

biorxiv.org
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Robert Battaglia retweeted
Gene-Wei Li Lab (@GeneWeiLiLab) profile picture
Gene-Wei Li Lab@GeneWeiLiLab22 Dec 2023
Excited to share our latest work from @bioSavinov, a K99 fellow in the lab (and corresponding author!). Andrew worked w/ @SassSeabass (@keating_lab) to develop FragFold: a massively parallel computational approach to discover inhibitory protein fragments! Check out his thread:
Andrew Savinov (@bioSavinov) profile pictureAndrew Savinov@bioSavinov22 Dec 2023
Want to design genetically encoded inhibitors of your favorite protein? Thrilled to announce our latest work w/ @SassSeabass, @keating_lab, & @GeneWeiLiLab, where we leverage massively parallel AlphaFold predictions to discover peptide inhibitors. biorxiv.org/content/10.1101/… (1/n)
Image from Andrew Savinov (@bioSavinov)
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Robert Battaglia retweeted
Gene-Wei Li Lab (@GeneWeiLiLab) profile picture
Gene-Wei Li Lab@GeneWeiLiLab15 Dec 2023
We are happy to share our latest work revealing that pseudouridine is found in bacterial mRNA! biorxiv.org/content/10.1101/…. A short thread: (1/n)
RluA is the major mRNA pseudouridine synthase in Escherichia coli

RluA is the major mRNA pseudouridine synthase in Escherichia coli

Pseudouridine (Ψ) is an ubiquitous RNA modification, present in the tRNAs and rRNAs of species across all domains of life. Conserved pseudouridine synthases modify the mRNAs of diverse eukaryotes,...

biorxiv.org
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Robert Battaglia retweeted
Gene-Wei Li Lab (@GeneWeiLiLab) profile picture
Gene-Wei Li Lab@GeneWeiLiLab18 Oct 2023
We are delighted to share a new method for live-cell transcriptome recording: Molecular Time Capsules! biorxiv.org/content/10.1101/… We envision MTCs will help reveal the origins of phenotypic heterogeneity, and facilitate the study of transcriptomes in hard-to-access locations. (1/n)
Molecular Time Capsules Enable Transcriptomic Recording in Living Cells

Molecular Time Capsules Enable Transcriptomic Recording in Living Cells

Live-cell transcriptomic recording can help reveal hidden cellular states that precede phenotypic transformation. Here we demonstrate the use of protein-based encapsulation for preserving samples of...

biorxiv.org
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Robert Battaglia retweeted
Gene-Wei Li Lab (@GeneWeiLiLab) profile picture
Gene-Wei Li Lab@GeneWeiLiLab15 May 2023
Congrats to Li lab graduate student Manraj Gill on receiving an MIT School of Science DEI Service Fellowship for his deep engagement in the mentorship of underrepresented students both within @MIT and in the broader community! @MITBiology @ScienceMIT
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What do the #epigenome and #Grogu have in common? Answer: the Force is strong in both of them. In our latest review (in press) with @dpmelters @NCIYaminiDalal, we discuss how mechanical forces shape chromatin structure, leading to biological functions. osf.io/s2h9j/
Image from Chieh-Ren (Jeremiah) Hsia (@JeremiahHsia)
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Congrats, Itai!
Itai Levin (@itai_levin) profile pictureItai Levin@itai_levin15 Dec 2022
I'm happy to share our work on computer-aided synthesis planning of hybrid enzymatic/synthetic reaction pathways with @Geneticdesigner and @cwcoley! nature.com/articles/s41467-0…
Merging enzymatic and synthetic chemistry with computational synthesis planning

Merging enzymatic and synthetic chemistry with computational synthesis planning

Nature Communications - The identification of synthetic routes combining enzymatic and non-enzymatic reactions has been challenging and requiring expert knowledge. Here, the authors describe a...

nature.com
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Beautiful structure and mechanism by @ch_hu1 and @AilongK lab!
Ailong Ke's Lab at Yale MB&B (@AilongK) profile pictureAilong Ke's Lab at Yale MB&B@AilongK25 Aug 2022
Caspases control programmed cell death in eukaryotes. Type III-E CRISPR harbors caspase-like proteases. Our new Science paper explains how Craspase switches on protease activity in RNA-guided fashion - safe alternatives to Cas nucleases in therapeutics. science.org/doi/10.1126/scie…
Craspase is a CRISPR RNA-guided, RNA-activated protease

Craspase is a CRISPR RNA-guided, RNA-activated protease

High-resolution mechanisms are established for RNA-guided RNA and protein cleavage by CRISPR-guided Caspase (Craspase).

science.org
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Way to go @aaronjoinerphd!! Awesome accomplishment by an awesome dude.
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IscB-ωRNA is as active as Cas9 in test tube, but only a fraction in size. Want to learn about their similarity and differences in high-res? What might be holding its activity back in human cells? Further miniaturization? Check out our new publication. science.org/doi/10.1126/scie…
Structural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9

Structural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9

The transition from ancestral IscB to Cas9 involved dwarfing the ωRNA and introducing protein domain replacements.

science.org
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Awesome and thought-provoking work by Lydia et al!
Lydia Herzel, AG Herzel, RNA Dynamics (@lh8314) profile pictureLydia Herzel, AG Herzel, RNA Dynamics@lh83149 May 2022
Check out our, @GeneWeiLiLab @julianstanley_, new paper showing that a large fraction of RNA molecules in bacteria are fragments of canonical full-length mRNAs with consequences for differential RNA-seq analysis: doi.org/10.1093/nar/gkac295

Ubiquitous mRNA decay fragments in E. coli redefine the functional transcriptome

Abstract. Bacterial mRNAs have short life cycles, in which transcription is rapidly followed by translation and degradation within seconds to minutes. The

academic.oup.com
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Robert Battaglia retweeted
Cornell MBG (@CornellMBG) profile picture
Cornell MBG@CornellMBG12 Apr 2022
Great feature on innovative research from the Ailong Ke lab! research.cornell.edu/news-fe…
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Robert Battaglia retweeted
Ronald Breaker (@RonBreaker) profile picture
Ronald Breaker@RonBreaker6 Apr 2022
Sad to learn that #Yale Prof. and colleague #SidneyAltman, 1989 Chemistry #Nobel recipient, has died. His publication on the #ribozyme action of #RNaseP was a milestone discovery in the now exploding field of #ncRNA. The photo shows him celebrating the news of his Nobel Prize.
Image from Ronald Breaker (@RonBreaker)
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The work of a great scientist and one of my best friends, @vera_rinaldi 🥳 It was always fun to make wild conjectures together as to what could be going on here! Congratulations!
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Thanks for interviewing me 😊 @WhiteheadInst
Whitehead Institute (@WhiteheadInst) profile pictureWhitehead Institute@WhiteheadInst27 Jan 2022
In the newest #WhiteheadPostdocProfiles, Jordana Bloom studies how hormonal changes during menopause affect gene expression. In December, she coordinated a Covid-safe lab cookie exchange — where lab members could flex their baking skills & stay connected: wi.mit.edu/news/meet-whitehe…
Image from Whitehead Institute (@WhiteheadInst)
Image from Whitehead Institute (@WhiteheadInst)
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Robert Battaglia retweeted
Ahmet Bakirbas (@plantbiol) profile picture
Ahmet Bakirbas@plantbiol18 Jan 2022
The latest Spotlight piece I've written for the @RNASociety featuring @RNABob . It was great to know Bob and learn about his exciting research on riboswitches!
The RNA Society (@RNASociety) profile pictureThe RNA Society@RNASociety5 Jan 2022
"It's important to keeping learning... you never know where the inspiration for a breakthrough could come from." Read more in this month's Spotlight featuring Dr. Robert Battaglia @RNABob, who is a post-doc in Gene-Wei Li's lab at @MIT! rnasociety.org/dr--robert-ba…
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Robert Battaglia retweeted
Helder Nakaya (@helder_nakaya) profile picture
Helder Nakaya
@helder_nakaya14 Jan 2022
75 years of research on human diseases in 1 minute
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Robert Battaglia retweeted
Sorek Lab (@SorekLab) profile picture
Sorek Lab@SorekLab14 Jan 2022
Pyroptosis is conserved between human and bacteria: caspase-activated gasdermins form pores and protect bacteria from phage Fantastic collaboration with the Kranzusch lab, now out in @ScienceMagazine Congrats @TanaWein & @algejohn ! science.org/doi/epdf/10.1126…
Image from Sorek Lab (@SorekLab)
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Robert Battaglia retweeted
Nils Walter Lab (@NilsWalterLab) profile picture
Nils Walter Lab@NilsWalterLab11 Jan 2022
Finally out-- Intimate look at how an anion can snap-lock a polyanionic riboswitch into a tight pseudoknot to upregulate bacterial #detox machinery: labor of love by postdocs Rajeev Yadav (now @michiganstateu), Julia Widom (now @uoregon) & Adrien Chauvier rdcu.be/cELsB
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Robert Battaglia retweeted
Andrew Savinov (@bioSavinov) profile picture
Andrew Savinov@bioSavinov6 Jan 2022
Thrilled to present our latest paper, up now on bioRxiv! biorxiv.org/content/10.1101/… Interested in mapping interaction interfaces of your favorite protein in vivo, or developing inhibitors of those interactions? Curious about regulation by small proteins/miniproteins? A thread (1/n)
Mapping functional regions of essential bacterial proteins with dominant-negative protein fragments

Mapping functional regions of essential bacterial proteins with dominant-negative protein fragments

Massively-parallel measurements of dominant negative inhibition by protein fragments have been used to map protein interaction sites and discover peptide inhibitors. However, the underlying princip...

biorxiv.org
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