Professor of Molecular Biology. RNA, chromatin and transcriptional regulation.
UCL, London
Joined May 2016
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Richard Jenner retweeted
New (to you?) preprint alert!
Can protein-coding mRNAs "moonlight" as nuclear-architectural RNAs?
Our revised preprint uncovers how nascent TTN RNA organizes a specialized nuclear compartment in cardiac cells—and how its disruption may contribute to heart disease. A thread! 🧵
We also found that G4 RNA preferentially binds to the inactivated form of PRC2. SAM and coactivator peptides inhibit G4 RNA binding through an allosteric mechanism, which is prevented by a SAM binding mutation. 7/8
John used cryo-EM to solve the structure of the monomeric PRC2 catalytic core bound to G4 RNA. He found that G4 RNA binds PRC2 through the disordered K and GR loops in EZH2. 3/8
Pleased to share our new pre-print revealing the mechanism through which G4 RNA binds and inhibits PRC2. Work led by John Houslay, a joint student between our group and the Wilson/Gamblin lab. 1/8
biorxiv.org/content/10.64898…
Richard Jenner retweeted
🧬 How does HP1α repress, and what happens when transcription is induced? Our new paper in @MolecularCell: HP1α stays bound, its barrier is local, and mouse chromocenters switch nanodomain by nanodomain. Paper: doi.org/10.1016/j.molcel.202… Thread with figures: bsky.app/profile/karsten-rip…
ALT Model scheme. Top, a silenced wild-type chromocenter with H3K9me3 nucleosomes and bound HP1α; VP16 is blocked, p65 and VPR activate it while HP1α and H3K9me3 stay. Bottom, a Suv39h dn chromocenter without HP1α, which p65 and VPR activate with acetylation and VP16 activates without it.
Richard Jenner retweeted
Incredibly happy to share the first publication from our lab, now published in Nature Cell Biology! 🎉
How do cells coordinate RNA processing with nuclear organization and the cell cycle?
nature.com/articles/s41556-0…
1/10
Richard Jenner retweeted
One of the (well, >1) problems in the lncRNA field is that some of the lncRNAs are not real genes, i.e., do not represent a standalone locus that produces independent transcripts. Some cases are more 🤔 than others, e.g. lncDACH that Matt Bennet from @bakergroupcvs dove into 🧵
Richard Jenner retweeted
This study identifies CpG islands at 3′UTRs as highly conserved, frequently methylated, and linked to cancer-related gene regulation. @Kanhere_Lab hubs.la/Q04qPckF0
Richard Jenner retweeted
Online Now: α-Satellite RNA maintains centromeric cohesion through R-loops dlvr.it/TTt08l
Richard Jenner retweeted
Excited to share the first pre-print from our lab!!
Check it out here! biorxiv.org/content/10.64898…
We found that many RNA-binding proteins understood to regulate RNA processing can also function like transcription factors and cofactors to directly regulate transcription.
Richard Jenner retweeted
Independent Senior Research Fellow position available! Working between UCL and UCLH, you will apply machine learning to digital pathology data and electronic health records to drive new insights in cancer biology and improve patient care.
To apply: lnkd.in/e9SX6WAT
Does PRC2 interact with RNA or is it an artefact? See our preprint showing how stringent denaturing purifications with spike-in controls support direct interaction between PRC2 and RNA in cells. biorxiv.org/content/10.64898…
ALT UV-crosslinked human cells containing Halo-tagged EZH2 were mixed with crosslinked mouse cells in which the protein was untagged and human EZH2 purified with Halo resin. Enrichment of human RNA (left) or mouse RNA (right) versus input was plotted against RNA abundance in the paired input sample. Windows with significant enrichment (p <10−6; binomial test) are shown in red. Human RNAs show enrichment versus input whereas mouse RNAs do not.
Richard Jenner retweeted
Now out in @NatureBiotech ! Do lncRNAs commonly bind 1000s of genomic sites? Maybe they do, but the dozens of studies that report genomic binding maps of lncRNAs are deeply flawed, with probes binding suprious DNA sites rather than RNA-bound ones. nature.com/articles/s41587-0…
A preprint‼️that's bound to ruffle some 🪶 "Widespread DNA off-targeting confounds studies of RNA chromatin occupancy" led by our @MicahGoldrich and Louis Delhaye from @pieter_mestdagh. TL;DR we show that many of lncRNA chromatin occupancy maps are flawed🧵biorxiv.org/content/10.1101/…
Richard Jenner retweeted
Computational analysis is playing an ever increasing role in the detection, management and understanding of cancer.
Equip yourself with the knowledge and skills to lead work in these areas with our new MSc in Computational Cancer, run with @UCLmedphys
ucl.ac.uk/prospective-studen…
Looking forward to hosting the next meeting of the London RNA Club on RNA Regulation in the Nucleus.
Wednesday 25th March at UCL.
Speakers including @BrockdorffLab, @Vichaico, @RobertVendramin and Folkert van Werven.
eventleaf.com/e/LondonRNAClu… to register.
