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Very proud to introduce ScisorWiz - a tool for visualizing long-read, single-cell differential isoform expression! It's easy to use and customizable based on individual needs! Huge thank yous to @noush_joglekar, @cheesepuuff, and @hagentilgner! academic.oup.com/bioinformat…
Alex Stein retweeted
I'm so excited to FINALLY present the latest preprint from the @hagentilgner lab where we look at alternative splicing in the brain across "space and time" biorxiv.org/content/10.1101/…
Alex Stein retweeted
Our preprint on single-cell long-read isoforms across mouse brain regions, developmental time points as well as human hippocampi is here:
biorxiv.org/content/10.1101/…
Great team work with contributions by many but led by @noush_joglekar, who invested almost 3 years into this.
Alex Stein retweeted
This tool is a godsend for anybody trying to visualize complex splicing patterns across multiple conditions. @astein7 did a fantastic job making it user friendly, intuitive, and with awesome interactive properties
We have been asked about our single-gene plots. The deal was 1) make huge coffee pot on Friday night; 2) while not good; do tweak bash code, then awk, then R for gene; done 3) plot ready on Saturday. Now @astein7 made it single-command: biorxiv.org/content/10.1101/…
Alex Stein retweeted
Our long-read plot library is out. Led by @astein7 with
help by @noush_joglekar and @cheesepuuff:
academic.oup.com/bioinformat…
1 command for Figs 4e in nature.com/articles/nbt.3242 2F in nature.com/articles/nbt.4259 5e in nature.com/articles/s41467-0… 5F in nature.com/articles/s41587-0… @PacBio @nanopore
Alex Stein retweeted
If you have seen any of our work, you know that we're extremely partial to pretty examples like this one ⬇️
Creating each one of these was always a frustrating task until @astein7 came along and made it a cakewalk. Try out his R package on your own data! academic.oup.com/bioinformat…
Alex Stein retweeted
ScisorWiz is out! New paper from the @hagentilgner lab with a tool to visualize single-cell isoform expression, by @astein7 with @noush_joglekar and @cheesepuuff
academic.oup.com/bioinformat…
Alex Stein retweeted
We have been asked about our single-gene plots. The deal was 1) make huge coffee pot on Friday night; 2) while not good; do tweak bash code, then awk, then R for gene; done 3) plot ready on Saturday. Now @astein7 made it single-command: biorxiv.org/content/10.1101/…
ScisorWiz is a tool for visualizing Differential Isoform Expression using single-cell RNA-sequencing data. The paper is up on bioRxiv! Extremely proud of our work here. Thanks to @noush_joglekar, @hagentilgner, and Chi-Lam! biorxiv.org/content/10.1101/…
Finally out: Our platform comparison for individual molecules:
genome.cshlp.org/content/ear…
Big congratulations to the members of the Tilgner Lab at WCM and all collaborators on this @NatureBiotech paper on single-nucleus isoform RNA sequencing (SnISOr-Seq)! nature.com/articles/s41587-0…
Alex Stein retweeted
I am beyond thrilled to present our work on single-nuclei isoform sequencing (SnISOr-Seq), now out in Nature Biotech nature.com/articles/s41587-0…. Thread below ⬇️
Alex Stein retweeted
Our new work on single-nuclei isoform sequencing is available on biorxiv: biorxiv.org/content/10.1101/… Led by postdocs @si_hardwick & Wen Hu and PhDstudent @noush_joglekar but with contributions by many. Applied to human PFC and sc splicing, TSS, polyA sites and combination patterns.
Alex Stein retweeted
Thought we'd start off '22 with a fresh serving of science on your timeline! 🧵
TL;DR we developed single-nuclei isoform sequencing in frozen human brain tissue and found some cool stuff: biorxiv.org/content/10.1101/…
I don't tweet often, but when I do, it is for a good reason! @noush_joglekar's hard-earned paper was published today and she was kind enough to include me in the list of authors and contributors! Congratulations to everyone involved!
nature.com/articles/s41467-0…
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Alex Stein retweeted
Immensely proud of work led by first grad student (@noush_joglekar), but with crucial contributions by so many (please do read the author list): nature.com/articles/s41467-0…
Single-cell isoform comparison of PFC and hippocampus and development of slide-isoform sequencing (Sl-ISO-Seq)
Alex Stein retweeted
Our labor of love is finally out! nature.com/articles/s41467-0…
A massive thank you to all our collaborators, without whom none of this would've been possible @ahmedElkoussy @bioinf_spbu @10xGenomics @SvenAT @bllapin @drisso1893 @erichjarvis @hagentilgner
Alex Stein retweeted
Excited to share work led by grad student Anoushka & important contribs by many. @noush_joglekar biorxiv.org/content/10.1101/…
Alex Stein retweeted
Excited to announce the newest preprint from the Tilgner lab - biorxiv.org/content/10.1101/…
We always knew that splicing is messy, especially at the single cell level, but we found that there's some method to the madness 1/n
