Simon Hardwick (@si_hardwick) profile picture

Simon Hardwick

@si_hardwick

RNA biology

 Melbourne
linkedin.com/in/simon-hardwick-ph-d-8979a128/
Joined November 2015
  • Tweets307
  • Following2K
  • Followers675
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Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)Photo by Simon Hardwick (@si_hardwick)
Replying to @naturemethods
@naturemethods has written a nice little synopsis of our recent SnISOr-Seq paper, including a chat with @hagentilgner: nature.com/articles/s41592-0…
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Only 2 days to go until the inaugural @ARNAconference kicks off in Thredbo! I'll be speaking in Session 2 on Monday morning 🧬 #arna #mrna
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Research Highlight by @DrLeiTang: SnISOr-Seq enables cell-type specific isoform analysis in frozen samples. Original paper: nature.com/articles/s41587-0… Highlight: nature.com/articles/s41592-0…
Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Nature Biotechnology - Complete RNA isoforms are captured by a new single-nuclei sequencing method.

nature.com
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Simon Hardwick retweeted
Liz Tseng (@Magdoll) profile picture
Liz Tseng@Magdoll11 Apr 2022
Hey there #Bioinformatics folks looking for a job, I have an academic PI friend looking to hire bioinfx talent to work on long read (@PacBio Iso-Seq) + other omics. International candidates welcome! no job req yet so for now DM me to be connected. RT appreciated ❤️
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Simon Hardwick retweeted
Oxford Nanopore (@nanopore) profile picture
Oxford Nanopore
@nanopore3 Apr 2022
.@si_hardwick & team present single-nuclei isoform RNA sequencing (SnISOr-Seq). Their method enables investigation of cell-type-specific splicing patterns & improves understanding of isoform combinations in healthy tissue & in disease: bit.ly/3wNrFvi
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Imagine you could sequence one molecule on @PacBio and @nanopore ... you could really tell all the differences on a single-molecule basis, where any discrepancy must be a mistake in one approach: genome.cshlp.org/content/ear… Led by the incredible Alla M. & Andrey P. @genomeresearch
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For the first time in the #covid pandemic, Australia now has more average daily cases than the US. Yet our average daily deaths is only 16, compared to 1,224 for the US 🤔 – at Sydney, New South Wales
We used ReadUntil targeted @nanopore sequencing to solve tricky tandem repeat disorders. Read all about it: science.org/doi/10.1126/scia…
Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted...

Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted...

Programmable targeted nanopore sequencing profiles all known pathogenic short tandem repeats (STRs) in a single test.

science.org
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Amazing work led by postdocs Simon H, Wen H and grad student Anoushka J, with contributions by so many : nature.com/articles/s41587-0… Get splicing info out of human brain. TSS-exon, exon-polyA and distant exon pairing rules are more cell-type specific. Adjacent exon pairs less so.
Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Nature Biotechnology - Complete RNA isoforms are captured by a new single-nuclei sequencing method.

nature.com
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Hot off the press! Latest paper from our lab is out now in Nature Biotech. Thanks to @noush_joglekar, @rainryuhei_wen, @hagentilgner and everyone else involved... nature.com/articles/s41587-0…
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Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue go.nature.com/3sNrrlc
Image from Nature Biotechnology (@NatureBiotech)
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Simon Hardwick retweeted
Anoushka (@noush_joglekar) profile picture
Anoushka@noush_joglekar7 Mar 2022
I am beyond thrilled to present our work on single-nuclei isoform sequencing (SnISOr-Seq), now out in Nature Biotech nature.com/articles/s41587-0…. Thread below ⬇️
Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Nature Biotechnology - Complete RNA isoforms are captured by a new single-nuclei sequencing method.

nature.com
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Had a blast leading this project with @noush_joglekar, @hagentilgner and many others at Cornell and elsewhere...
Anoushka (@noush_joglekar) profile pictureAnoushka@noush_joglekar1 Jan 2022
Thought we'd start off '22 with a fresh serving of science on your timeline! 🧵 TL;DR we developed single-nuclei isoform sequencing in frozen human brain tissue and found some cool stuff: biorxiv.org/content/10.1101/…
Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across...

Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across...

Single-nuclei RNA-Seq is being widely employed to investigate cell types, especially of human brain and other frozen samples. In contrast to single-cell approaches, however, the majority of single-...

biorxiv.org
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Simon Hardwick retweeted
bioRxiv (@biorxivpreprint) profile picture
bioRxiv@biorxivpreprint31 Dec 2021
Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across human brain cell types biorxiv.org/cgi/content/shor… #bioRxiv
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Our new work on single-nuclei isoform sequencing is available on biorxiv: biorxiv.org/content/10.1101/… Led by postdocs @si_hardwick & Wen Hu and PhDstudent @noush_joglekar but with contributions by many. Applied to human PFC and sc splicing, TSS, polyA sites and combination patterns.
Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across...

Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across...

Single-nuclei RNA-Seq is being widely employed to investigate cell types, especially of human brain and other frozen samples. In contrast to single-cell approaches, however, the majority of single-...

biorxiv.org
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Our new preprint is up! We develop and apply a new technique which enables single-nuclei, long-read RNA sequencing in human brain samples (or any frozen, archived, or hard-to-dissociate sample) @hagentilgner biorxiv.org/content/10.1101/…
Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across...

Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across...

Single-nuclei RNA-Seq is being widely employed to investigate cell types, especially of human brain and other frozen samples. In contrast to single-cell approaches, however, the majority of single-...

biorxiv.org
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Simon Hardwick retweeted
Feng Zhang (@zhangf) profile picture
Feng Zhang
@zhangf19 Aug 2021
We are really excited to share our work on a new modular RNA delivery technology called SEND, built from endogenous components found in the mammalian genome. @ScienceMagazine science.sciencemag.org/conte…
Image from Feng Zhang (@zhangf)
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255,964 #covid19 vaccine doses given yesterday in Australia. Scaled for population, this is the equivalent of ~3.4 million doses in the US, which they were hitting in late March. Not bad Australia, but we can still do much better!
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Very troubling that the virus is in IKEA, as viruses know a thing or two about self-assembly #covidnsw
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Johnson & Johnson's other bestsellers: band-aids, tylenol and baby powder 💉
Hot off the press from our lab!
Hagen Tilgner / @hagentilgner@genomic.social (@hagentilgner) profile pictureHagen Tilgner / @[email protected]@hagentilgner19 Jan 2021
Immensely proud of work led by first grad student (@noush_joglekar), but with crucial contributions by so many (please do read the author list): nature.com/articles/s41467-0… Single-cell isoform comparison of PFC and hippocampus and development of slide-isoform sequencing (Sl-ISO-Seq)
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